G protein coupled receptors (GPCRs)
Sources of DNA Damage
|ROS||abasic sites, DNA crosslinks, base modifications (bulky and non-bulky)||NER, BER|
|Replication Errors||Incorrectly paired nucleotides||DNA Pol proofreading, MMR|
|– UV-A||Creation of free radicals (indirect damage)|
|– UV-B||Creation of primer dimers, crosslinking between adjacent C&T|
|Radiation||Double strand breaks||NHEJ, HR|
|Hydrolysis||deamination, abasic sites||NER, BER|
|Toxins||DNA adducts, crosslinking, oxidized bases, alkylated phosphotriesters||BER, NER|
Cell Cycle Phases
Proteins included are for yeast.
G1 is the phase after cell division. This phase begins with non cyclins present in the cell. This is important as it allows for the creation of the pre-replicative complex (pre-RC) at the origins of replication in DNA. The pre-RC complex consists of the ORC (origin recognition complex – a six unit complex) recruitment to the ORC site in the DNA. The ORC protein recruits Cdc6 (cell division control protein 6 homolog protein) and cdt1 in complex. These proteins are then important in loading mcm2-7 (mini chromosome maintenance proteins 2-7).